A study of the applicability of software-defined networking in industrial networks

173 p.Las redes industriales interconectan sensores y actuadores para llevar a cabo funciones de monitorización, control y protección en diferentes entornos, tales como sistemas de transporte o sistemas de automatización industrial. Estos sistemas ciberfísicos generalmente están soportados por múltiples redes de datos, ya sean cableadas o inalámbricas, a las cuales demandan nuevas prestaciones, de forma que el control y gestión de tales redes deben estar acoplados a las condiciones del propio sistema industrial. De este modo, aparecen requisitos relacionados con la flexibilidad, mantenibilidad y adaptabilidad, al mismo tiempo que las restricciones de calidad de servicio no se vean afectadas. Sin embargo, las estrategias de control de red tradicionales generalmente no se adaptan eficientemente a entornos cada vez más dinámicos y heterogéneos.Tras definir un conjunto de requerimientos de red y analizar las limitaciones de las soluciones actuales, se deduce que un control provisto independientemente de los propios dispositivos de red añadiría flexibilidad a dichas redes. Por consiguiente, la presente tesis explora la aplicabilidad de las redes definidas por software (Software-Defined Networking, SDN) en sistemas de automatización industrial. Para llevar a cabo este enfoque, se ha tomado como caso de estudio las redes de automatización basadas en el estándar IEC 61850, el cual es ampliamente usado en el diseño de las redes de comunicaciones en sistemas de distribución de energía, tales como las subestaciones eléctricas. El estándar IEC 61850 define diferentes servicios y protocolos con altos requisitos en terminos de latencia y disponibilidad de la red, los cuales han de ser satisfechos mediante técnicas de ingeniería de tráfico. Como resultado, aprovechando la flexibilidad y programabilidad ofrecidas por las redes definidas por software, en esta tesis se propone una arquitectura de control basada en el protocolo OpenFlow que, incluyendo tecnologías de gestión y monitorización de red, permite establecer políticas de tráfico acorde a su prioridad y al estado de la red.Además, las subestaciones eléctricas son un ejemplo representativo de infraestructura crítica, que son aquellas en las que un fallo puede resultar en graves pérdidas económicas, daños físicos y materiales. De esta forma, tales sistemas deben ser extremadamente seguros y robustos, por lo que es conveniente la implementación de topologías redundantes que ofrezcan un tiempo de reacción ante fallos mínimo. Con tal objetivo, el estándar IEC 62439-3 define los protocolos Parallel Redundancy Protocol (PRP) y High-availability Seamless Redundancy (HSR), los cuales garantizan un tiempo de recuperación nulo en caso de fallo mediante la redundancia activa de datos en redes Ethernet. Sin embargo, la gestión de redes basadas en PRP y HSR es estática e inflexible, lo que, añadido a la reducción de ancho de banda debida la duplicación de datos, hace difícil un control eficiente de los recursos disponibles. En dicho sentido, esta tesis propone control de la redundancia basado en el paradigma SDN para un aprovechamiento eficiente de topologías malladas, al mismo tiempo que se garantiza la disponibilidad de las aplicaciones de control y monitorización. En particular, se discute cómo el protocolo OpenFlow permite a un controlador externo configurar múltiples caminos redundantes entre dispositivos con varias interfaces de red, así como en entornos inalámbricos. De esta forma, los servicios críticos pueden protegerse en situaciones de interferencia y movilidad.La evaluación de la idoneidad de las soluciones propuestas ha sido llevada a cabo, principalmente, mediante la emulación de diferentes topologías y tipos de tráfico. Igualmente, se ha estudiado analítica y experimentalmente cómo afecta a la latencia el poder reducir el número de saltos en las comunicaciones con respecto al uso de un árbol de expansión, así como balancear la carga en una red de nivel 2. Además, se ha realizado un análisis de la mejora de la eficiencia en el uso de los recursos de red y la robustez alcanzada con la combinación de los protocolos PRP y HSR con un control llevado a cabo mediante OpenFlow. Estos resultados muestran que el modelo SDN podría mejorar significativamente las prestaciones de una red industrial de misión crítica

La imagen y la narrativa como herramientas para el abordaje psicosocial en escenarios de violencia. Departamento Bolívar y Cesar

El enfoque narrativo en psicología contemporánea se ha revelado como una herramienta valiosa para abordar la violencia sistémica, centrándose en las historias individuales como vehículo para comprender experiencias y construir significados. Este documento busca proponer estrategias de afrontamiento psicosocial frente al sufrimiento provocado por la violencia, explorando eventos traumáticos desde una perspectiva narrativa. La representación visual y las narrativas juegan un papel esencial en la construcción de la memoria individual y colectiva en contextos afectados por el conflicto armado en Colombia. Se analiza específicamente el caso de la Masacre en El Salado, revelando la incertidumbre y desamparo de las víctimas, cuyas historias a menudo quedan sepultadas en el silencio estatal. El estudio aborda los impactos desde una perspectiva bio-psico-socio-cultural, reconociendo la complejidad de los factores que influyen en la experiencia de la violencia. Se exploran los elementos simbólicos asociados con la violencia, la resiliencia y las experiencias de transformación. A pesar de las profundas cicatrices, se destaca la resiliencia y los procesos de transformación de la comunidad en busca de una mejor calidad de vida, enfocándose en la realización del proyecto de vida como crucial para la recuperación. Además, el Informe de la narrativa-foto voz agrega una capa visual para expresar las experiencias y emociones, enriqueciendo la comprensión de la complejidad de la situación. En resumen, se aborda la violencia desde una perspectiva narrativa y simbólica, destacando la importancia de la resiliencia y los procesos de transformación en la búsqueda de la recuperación.The narrative approach in contemporary psychology has emerged as a valuable tool to address systemic violence, focusing on individual stories as a vehicle to understand experiences and construct meanings. This document seeks to propose psychosocial coping strategies against the suffering caused by violence, exploring traumatic events from a narrative perspective. Visual representation and narratives play an essential role in the construction of individual and collective memory in contexts affected by the armed conflict in Colombia. The case of the El Salado Massacre is specifically analyzed, revealing the uncertainty and helplessness of the victims, whose stories are often buried in state silence. The study addresses the impacts from a bio-psycho-socio-cultural perspective, recognizing the complexity of the factors that influence the experience of violence. The symbolic elements associated with violence, resilience and transformation experiences are explored. Despite the deep scars, the resilience and transformation processes of the community in search of a better quality of life stand out, focusing on the realization of the life project as crucial for recovery. Additionally, the Voice Photo Narrative Report adds a visual layer to express experiences and emotions, enriching the understanding of the complexity of the situation. In summary, violence is addressed from a narrative and symbolic perspective, highlighting the importance of resilience and transformation processes in the search for recovery

La imagen y la narrativa como herramientas para el abordaje psicosocial en escenarios de violencia. Departamento Bolívar y Cesar

El enfoque narrativo en psicología contemporánea se ha revelado como una herramienta valiosa para abordar la violencia sistémica, centrándose en las historias individuales como vehículo para comprender experiencias y construir significados. Este documento busca proponer estrategias de afrontamiento psicosocial frente al sufrimiento provocado por la violencia, explorando eventos traumáticos desde una perspectiva narrativa. La representación visual y las narrativas juegan un papel esencial en la construcción de la memoria individual y colectiva en contextos afectados por el conflicto armado en Colombia. Se analiza específicamente el caso de la Masacre en El Salado, revelando la incertidumbre y desamparo de las víctimas, cuyas historias a menudo quedan sepultadas en el silencio estatal. El estudio aborda los impactos desde una perspectiva bio-psico-socio-cultural, reconociendo la complejidad de los factores que influyen en la experiencia de la violencia. Se exploran los elementos simbólicos asociados con la violencia, la resiliencia y las experiencias de transformación. A pesar de las profundas cicatrices, se destaca la resiliencia y los procesos de transformación de la comunidad en busca de una mejor calidad de vida, enfocándose en la realización del proyecto de vida como crucial para la recuperación. Además, el Informe de la narrativa-foto voz agrega una capa visual para expresar las experiencias y emociones, enriqueciendo la comprensión de la complejidad de la situación. En resumen, se aborda la violencia desde una perspectiva narrativa y simbólica, destacando la importancia de la resiliencia y los procesos de transformación en la búsqueda de la recuperación.The narrative approach in contemporary psychology has emerged as a valuable tool to address systemic violence, focusing on individual stories as a vehicle to understand experiences and construct meanings. This document seeks to propose psychosocial coping strategies against the suffering caused by violence, exploring traumatic events from a narrative perspective. Visual representation and narratives play an essential role in the construction of individual and collective memory in contexts affected by the armed conflict in Colombia. The case of the El Salado Massacre is specifically analyzed, revealing the uncertainty and helplessness of the victims, whose stories are often buried in state silence. The study addresses the impacts from a bio-psycho-socio-cultural perspective, recognizing the complexity of the factors that influence the experience of violence. The symbolic elements associated with violence, resilience and transformation experiences are explored. Despite the deep scars, the resilience and transformation processes of the community in search of a better quality of life stand out, focusing on the realization of the life project as crucial for recovery. Additionally, the Voice Photo Narrative Report adds a visual layer to express experiences and emotions, enriching the understanding of the complexity of the situation. In summary, violence is addressed from a narrative and symbolic perspective, highlighting the importance of resilience and transformation processes in the search for recovery

Isogenic GAA-KO Murine Muscle Cell Lines Mimicking Severe Pompe Mutations as Preclinical Models for the Screening of Potential Gene Therapy Strategies

Pompe disease (PD) is a rare disorder caused by mutations in the acid alpha-glucosidase (GAA) gene. Most gene therapies (GT) partially rely on the cross-correction of unmodified cells through the uptake of the GAA enzyme secreted by corrected cells. In the present study, we generated isogenic murine GAA-KO cell lines resembling severe mutations from Pompe patients. All of the generated GAA-KO cells lacked GAA activity and presented an increased autophagy and increased glycogen content by means of myotube differentiation as well as the downregulation of mannose 6-phosphate receptors (CI-MPRs), validating them as models for PD. Additionally, different chimeric murine GAA proteins (IFG, IFLG and 2G) were designed with the aim to improve their therapeutic activity. Phenotypic rescue analyses using lentiviral vectors point to IFG chimera as the best candidate in restoring GAA activity, normalising the autophagic marker p62 and surface levels of CI-MPRs. Interestingly, in vivo administration of liver-directed AAVs expressing the chimeras further confirmed the good behaviour of IFG, achieving cross-correction in heart tissue. In summary, we generated different isogenic murine muscle cell lines mimicking the severe PD phenotype, as well as validating their applicability as preclinical models in order to reduce animal experimentation.Fundacion Poco Frecuente (Almeria)Asociacion Espanola de Enfermos de Glucogenosis (AEEG)Asociacion Espanola de Enfermos de Pompe (AEEP

Ionospheric response modeling under eclipse conditions: Evaluation of 14 December 2020, total solar eclipse prediction over the South American sector

In this work, we evaluate the SUPIM-INPE model prediction of the 14 December 2020, total solar eclipse over the South American continent. We compare the predictions with data from multiple instruments for monitoring the ionosphere and with different obscuration percentages (i.e., Jicamarca, 12.0°S, 76.8°W, 17%; Tucumán 26.9°S, 65.4° W, 49%; Chillán 36.6°S, 72.0°W; and Bahía Blanca, 38.7°S, 62.3°W, reach 95% obscuration) due to the eclipse. The analysis is done under total eclipse conditions and non-total eclipse conditions. Results obtained suggest that the model was able to reproduce with high accuracy both the daily variation and the eclipse impacts of E and F1 layers in the majority of the stations evaluated (except in Jicamarca station). The comparison at the F2 layer indicates small differences (<7.8%) between the predictions and observations at all stations during the eclipse periods. Additionally, statistical metrics reinforce the conclusion of a good performance of the model. Predicted and calibrated Total Electron Content (TEC, using 3 different techniques) are also compared. Results show that, although none of the selected TEC calibration methods have a good agreement with the SUPIM-INPE prediction, they exhibit similar trends in most of the cases. We also analyze data from the Jicamarca Incoherent Scatter Radar (ISR), and Swarm-A and GOLD missions. The electron temperature changes observed in ISR and Swarm-A are underestimated by the prediction. Also, important changes in the O/N2 ratio due to the eclipse, have been observed with GOLD mission data. Thus, future versions of the SUPIM-INPE model for eclipse conditions should consider effects on thermospheric winds and changes in composition, specifically in the O/N2 ratio

Evolution of the use of corticosteroids for the treatment of hospitalised COVID-19 patients in Spain between March and November 2020: SEMI-COVID national registry

Objectives: Since the results of the RECOVERY trial, WHO recommendations about the use of corticosteroids (CTs) in COVID-19 have changed. The aim of the study is to analyse the evolutive use of CTs in Spain during the pandemic to assess the potential influence of new recommendations. Material and methods: A retrospective, descriptive, and observational study was conducted on adults hospitalised due to COVID-19 in Spain who were included in the SEMI-COVID- 19 Registry from March to November 2020. Results: CTs were used in 6053 (36.21%) of the included patients. The patients were older (mean (SD)) (69.6 (14.6) vs. 66.0 (16.8) years; p < 0.001), with hypertension (57.0% vs. 47.7%; p < 0.001), obesity (26.4% vs. 19.3%; p < 0.0001), and multimorbidity prevalence (20.6% vs. 16.1%; p < 0.001). These patients had higher values (mean (95% CI)) of C-reactive protein (CRP) (86 (32.7-160) vs. 49.3 (16-109) mg/dL; p < 0.001), ferritin (791 (393-1534) vs. 470 (236- 996) µg/dL; p < 0.001), D dimer (750 (430-1400) vs. 617 (345-1180) µg/dL; p < 0.001), and lower Sp02/Fi02 (266 (91.1) vs. 301 (101); p < 0.001). Since June 2020, there was an increment in the use of CTs (March vs. September; p < 0.001). Overall, 20% did not receive steroids, and 40% received less than 200 mg accumulated prednisone equivalent dose (APED). Severe patients are treated with higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%. Conclusions: Patients with greater comorbidity, severity, and inflammatory markers were those treated with CTs. In severe patients, there is a trend towards the use of higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Gestión del conocimiento. Perspectiva multidisciplinaria. Volumen 9

El libro “Gestión del Conocimiento. Perspectiva Multidisciplinaria”, volumen 9, de la Colección Unión Global, es resultado de investigaciones. Los capítulos del libro, son resultados de investigaciones desarrolladas por sus autores. El libro es una publicación internacional, seriada, continua, arbitrada de acceso abierto a todas las áreas del conocimiento, que cuenta con el esfuerzo de investigadores de varios países del mundo, orientada a contribuir con procesos de gestión del conocimiento científico, tecnológico y humanístico que consoliden la transformación del conocimiento en diferentes escenarios, tanto organizacionales como universitarios, para el desarrollo de habilidades cognitivas del quehacer diario. La gestión del conocimiento es un camino para consolidar una plataforma en las empresas públicas o privadas, entidades educativas, organizaciones no gubernamentales, ya sea generando políticas para todas las jerarquías o un modelo de gestión para la administración, donde es fundamental articular el conocimiento, los trabajadores, directivos, el espacio de trabajo, hacia la creación de ambientes propicios para el desarrollo integral de las instituciones

Spatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions

Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41–6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain.Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000–2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method in BEAST v1.8.0 and by reconstructing ancestral states using the criterion of parsimony in Mesquite v3.4, respectively.Results: The HIV-1 CRF02_AG prevalence differed across Spanish autonomous communities we sampled from (p &lt; 0.001). Phylogenetic analysis revealed that 52.7% of the CRF02_AG sequences formed 56 monophyletic clusters, with a range of 2–79 sequences. The CRF02_AG regional dispersal differed across Spain (p = 0.003), as suggested by monophyletic clustering. For the largest monophyletic cluster (subepidemic) (N = 79), 49.4% of the clustered sequences originated from Madrid, while most sequences (51.9%) had been obtained from men having sex with men (MSM). Molecular clock analysis suggested that the origin (tMRCA) of the CRF02_AG subepidemic was in 2002 (median estimate; 95% Highest Posterior Density-HPD interval: 1999–2004). Additionally, we found significant clustering within the CRF02_AG subepidemic according to the ethnic origin.Conclusion: CRF02_AG has been introduced as a result of multiple introductions in Spain, following regional dispersal in several cases. We showed that CRF02_AG transmissions were mostly due to regional dispersal in Spain. The hot-spot for the largest CRF02_AG regional subepidemic in Spain was in Madrid associated with MSM transmission risk group. The existence of subepidemics suggest that several spillovers occurred from Madrid to other areas. CRF02_AG sequences from Hispanics were clustered in a separate subclade suggesting no linkage between the local and Hispanic subepidemics

Differential regulation of insulin signalling by monomeric and oligomeric amyloid beta-peptide

Alzheimer's disease and Type 2 diabetes are pathological processes associated to ageing. Moreover, there are evidences supporting a mechanistic link between Alzheimer's disease and insulin resistance (one of the first hallmarks of Type 2 diabetes). Regarding Alzheimer's disease, amyloid β-peptide aggregation into β-sheets is the main hallmark of Alzheimer's disease. At monomeric state, amyloid β-peptide is not toxic but its function in brain, if any, is unknown. Here we show, by in silico study, that monomeric amyloid β-peptide 1-40 shares the tertiary structure with insulin and is thereby able to bind and activate insulin receptor. We validated this prediction experimentally by treating human neuroblastoma cells with increasing concentrations of monomeric amyloid β-peptide 1-40. Our results confirm that monomeric amyloid β-peptide 1-40 activates insulin receptor autophosphorylation, triggering downstream enzyme phosphorylations and the glucose Transporter 4 translocation to the membrane. On the other hand, neuronal insulin resistance is known to be associated to Alzheimer's disease since early stages. We thus modelled the docking of oligomeric amyloid β-peptide 1-40 to insulin receptor. We found that oligomeric amyloid β-peptide 1-40 blocks insulin receptor, impairing its activation. It was confirmed in vitro by observing the lack of insulin receptor autophosphorylation, and also the impairment of insulin-induced intracellular enzyme activations and the glucose Transporter 4 translocation to the membrane. By biological system analysis, we have carried out a mathematical model recapitulating the process that turns amyloid β-peptide binding to insulin receptor from the physiological to the pathophysiological regime. Our results suggest that monomeric amyloid β-peptide 1-40 contributes to mimic insulin effects in the brain, which could be good when neurons have an extra requirement of energy beside the well-known protective effects on insulin intracellular signalling, while its accumulation and subsequent oligomerization blocks the insulin receptor producing insulin resistance and compromising neuronal metabolism and protective pathways.This work was supported by the Spanish Ministry of Science and Innovation and Agencia Estatal de Investigación plus European Regional Development Fund (FEDER Funds) through grants PID2020-117691RB-I00/AEI/10.13039/501100011033 (F.J.M.), SAF2017-83372-R (F.J.M.), BIO2017-85329-R (B.O.), PGC2018-101251-B-I00 (J.G.-O.) and RTI2018-094579-B-I00 (X.F.-B.). This work was also funded by the Spanish Institute of Health Carlos III by project reference AC20/00009 -FEDER/UE and European Research Era Net (ERANET) ERA-CVD_JTC2020-015 (J.G.-O.), by the ‘María de Maeztu Programme’ for Units of Excellence in Research and Development (R&D; award CEX2018-000792-M), Generalitat de Catalunya (Spain) through the grant 2017-SGR-908 (X.F.-B.) and Fundación QUAES through Cátedra QUAES-UPF de Biomedicina e Ingeniería Biomédica. The Instituto de Salud Global de Barcelona (ISGlobal) and Institute for Bioengineering of Catalonia (IBEC) are members of the Centres de Recerca de Catalunya (CERCA) Programme, Generalitat de Catalunya (Spain). We acknowledge support from the Spanish Ministry of Science, Innovation and Universities through the ‘Centro de Excelencia Severo Ochoa 2019–2023’ Programme (CEX2018-000806-S). This research is part of ISGlobal's Programme on the Molecular Mechanisms of Malaria, which is partially supported by the Fundación Ramón Areces. J.G.-O. also acknowledges support from the Institució Catalana de Recerca i Estudis Avançats (ICREA) Academia programme